188宝金博页面版

净水技术 2021,40( s2 ) : 84-88 ,93 Water Purification Technology匚 t 海城投科创与实践专目韩小蒙.秋冬季节抗性基因在 AA0 反应器生物段的去除和水平转移过程 ［ J ］ . 净水技术, 2021, 40(s2) ： 84-88, 93.HAN X M. Removal and horizontal transfer of ARGs in biological treatment unit of AAO reactor in autumn and winter ［ J ］ . Water Pu ­rification Technology, 2021, 40(s2): 84 - 8 & 93.秋冬季节抗性基因在 AAO 反应器生物段的去除和水平转移过程韩小蒙(上海城市水资源开发利用国家工程中心有限公司 ， 上海 200082 )摘要厌氧-缺氧-好氧 ( anaerobic-anoxic-oxic , AAO ) 反应器的活性污泥中具有高密度的微生物 ， 抗性基 S(antibiotic resis tance genes,ARGs) 汇入后可能发生水平转移并向水体释放 。 因此,文中考察了不同进水流量下 AAO 反应器在秋季和冬季对 ARGs 的去除效果及 ARGs 水平转移过程 。 监测结果显 7K, 秋季进水 ARGs 浓度低于冬季 ， 相应的高流量组 (high-flow reactor, HFR) 和低流量组 (low-flow reactor,LFR ) tH 水中 sull.tetX.ermR.intll 和 ］ 6S rDNA 浓度也低于冬季 。 秋季 LFR 出水 ARGs 浓度 高于 HFR, 冬季两者近似 。 这反映了季节和进水流量会影响 AAO 反应器对 ARGs 的去除 。 两组反应器在秋季和冬季对不同种 类的 ARGs 对数去除率在 0.72-1.85 log 。 值得注意的是 ， 虽然秋冬季节两组反应器的出水 ARGs 浓度均低于进水 ， 但是 LFR 出 水中 sullMX 和 bla^ 的相对丰度却高于进水 ， 推测部分 ARGs 可能发生了向无抗性菌株水平转移的过程 。 因此 ， 从控制 ARGs 排放的角度 ， 需对 AAO 反应器出水进行消毒等处理 ， 以进一步降低 ARGs 浓度与其相对丰度 。关键词抗性基因 AAO 反应器秋冬季节生物段处理水平转移中图分类号 :X172 文献标识码: A 文章编号: 1009-0177(2021 )s2-0084-06DOI: 10. 15890/j. cnki.jsjs. 2021. s2. 018Removal and Horizontal Transfer of ARGs in Biological Treatment Unit of AAO Reactor in Autumn and WinterHAN Xiaomeng(Shanghai Urban Water Resources Development and Utilization National Engineering Center Co., Ltd., Shanghai 200082, China)Abstract Since there exists high density of microorganisms in activated sludge in anaerobic-anoxic-oxic (AAO) reactor, horizontal transfer and spread into the water environment of antibiotic resistance genes (ARGs) may occur after they import the AAO reactor. Therefore, in this study the removal effect and horizontal transfer of ARGs in the AAO reactor with different inflow rate were investi ­gated in the autumn and winter. Results showed that the ARG concentration of inflow was lower in the autumn than that in the winter. Correspondingly, the effluent concentration of sull, ieiX, emzB, intll and 16S rDNA of both the high — flow reactor (HFR) and low-flow reactor (LFR) was lower in autumn than that in the winter. In autumn, effluent concentration of ARGs in LFR exceeded that in HFR while in winter the effluent concentration of HFR and LFR was similar. This reflected that the season and inflow rate affected ARG removal in the AAO reactor. Removal efficiency of different kinds of ARGs ranged from 0.72 to 1.85 log in HFR and LFR in different seasons. It should be noticed that although the ARG concentration of effluent was lower than that of inflow in both seasons, the rela ­tive abundance of sull, tetX. and bla^ of effluent became higher than that of inflow. It might be inferred that parts of ARGs horizon ­tally transferred to some bacterial strains without antibiotic resistance. From the aspect of controlling ARGs discharge, disinfection was needed for the effluent of AAO reactors to reduce ARGs concentration and relative abundance.Keywords antibiotic resistance genes AAO reactor autumn and winter biological treatment horizontal transfer［ 收稿日期 ］ 2021-09-26［ 基金项目 ］ 上海市青年科技英才扬帆计划 (19YF1415000 ) ; 上海城投(集团 ) 有限公司科技创新计划项 KCTKY-PTZX-2020-017 ) ［ 作者简介 ］ 韩小蒙 (1988_ ) ， 女,高级工程师 , 研究方向污水污泥处理与资源化 , E-mail : hanxiaomeng@tongji.edu.cn 0— 84 —。 。. .。 。