188宝金博页面版

CMYK临床皮肤科杂志2025年54卷第6期J Clin Dermatol,June 2025,Vol.54,No.6皮肤基底细胞癌中TBX1的表达及其调控细胞增殖的生物学机制331论著时永强，范治强，李文超，赵云超宝鸡市人民医院整形美容科，陕西　宝鸡721000[摘要】目的：研究皮肤基底细胞癌(BBC)T盒转录因子（TBX)1的表达及其调控细胞增殖的生物学机制。方法：选取手术切除的皮肤BCC组织和正常皮肤组织,检测TBX1的mRNA和蛋白相对表达水平。培养BCC细胞株TE354T,分组并分别转染阴性对照(NC)小干扰RNA(siRNA）、T BX1siRNA、空白载体、Sonic hedgehog(Shh)过表达载体及Gli家族锌指（Gli)2过表达载体，用不同浓度(1μmol/L、5umol/L、10μmol/L)Sh抑制剂、不同浓度(5μmol/L、10 μmol/L20μmol/L)Cli2抑制剂处理，检测细胞增殖的吸光度(A）调亡率及TBX1、Shh、G lil、G li2的mRNA和蛋白水平。结果：皮肤BCC组织中TBX1的mRNA表达水平和蛋白水平均高于正常皮肤组织（P<0.05)；与si-NC组比较,si-TBX1组TE354T细胞24h、48h、72h的A值及细胞中TBX1、Shh、G li2的表达水平降低(P<0.05),细胞调亡率增加(P<0.05),Gli1的表达水平差异无统计学意义(P>0.05)；不同浓度Shh抑制剂组、Gli2抑制剂组TE354T细胞24h、48h、72h的A均显著降低(P<0.05)；与si-TBX1+空白质粒组比较,si-TBX1+Shh质粒组和si-TBX1+Gli2质粒组TE354T细胞24h、48h、72h的A值均增加,细胞调亡率降低(P<0.05）。结论：皮肤BCC中TBX1表达增加,敲低TBX1通过抑制Shh/Gli2通路抑制皮肤BCC细胞株TE354增殖。[关键词】皮肤基底细胞癌；T盒转录因子1；Sonic hedgehog；G li家族锌指2；细胞增殖[中图分类号] R739.5doi:10.16761/j.cnki.1000-4963.2025.06.004Expression and biological mechanism of TBX1 in skin basal cell carcinomaSHI Yongqiang, FAN Zhiqiang, LI Wenchao, ZHAO YunchaoDepartment of Plastic Surgery and Beauty, BaoJi People's Hospital, BaoJi 721000, China[Abstract] Objective: To investigate the expression of T-box transcription factor 1(TBX1) in skin basal cell carcinoma(BCC)and its biological mechanism of regulating BCC cell proliferation. Methods: Skin BCC and adjacent normal tissues were col-lected from surgical specimens in our hospital from January 2020 to December 2023, and the relative mRNA and protein ex-pression levels of TBX1 were detected. Skin BCC cell line TE354T was cultured, grouped and transfected with negative con-trol(NC), small interfering RNA(siRNA), TBX1 siRNA, blank vector, Sonic hedgehog(Shh) overexpression vector, Gli familyzinc finger(Gli) 2 overexpression vector, respectively. Then, the cells were treated with different concentrations of Shh in-hibitors(1 μmol/L, 5 μmol/L, 10 μmol/L) and Gli2 inhibitors(5 μmol/L, 10 μmol/L, 20 μmol/L). The absorbance value(A) ofcell proliferation, apoptosis rate, and relative mRNA and protein expression levels of TBX1, Sh, Glil and Gli2 were detect-ed. Results: The relative mRNA and protein expression levels of TBX1 in skin BCC tissues were higher than those in nor-mal skin tissues(P<0.05). Compared with the si-NC group, the A values at 24 h, 48h and 72h and the relative expressionlevels of TBX1, Shh and Gli2 in TE354T cells in the si-TBX1 group were decreased(P<0.05). The cell apoptosis rate in thesi-TBX1 group was increased(P<0.05), while the relative expression level of Glil had no significant diference(P>0.05). TheA values at 24h, 48h and 72h of TE354T cells in the Shh inhibitor group and Gli2 inhibitor group were significantly de-creased(P<0.05). Compared with the si-TBX1+blank plasmid group, the A values at 24 h, 48 h and 72 h of TE354T cellsin the si-TBX1+Shh plasmid group and the si-TBX1+Gli2 plasmid group increased, and the cell apoptosis rate were decreased(P<0.05). Conclusion: TBX1 expression increases in skin BCC, and TBX1 knockdown inhibits the proliferation of skin BCCcell line TE354T by inhibiting the Shh/Gli2 pathway.[Key words] skin basal cell carcinoma; T-box transcription factor l; Sonic hedgehog; Gli family zinc finger 2; proliferation[J Clin Dermatol, 2025, 54(6):331-337][文献标识码] A [文章编号]1000-4963(2025)06-0331-07皮肤基底细胞癌(basal cell carcinoma,BCC)是最收稿日期：2024-05-23；修回日期：2024-11-29通信作者：赵云超,Email:1107794108@qq.com常见的非黑素瘤性皮肤肿瘤,手术切除是BCC 的主要治疗方法[-2]。目前,BCC 的发病机制不明,临床上也缺乏有效的靶向治疗手段。T盒转录因子(T-box transcrip-tion factor,TBX)1 是一种调控胚胎发育、组织生长和分