Corporate HeadquartersAssociates of Cape Cod, Inc.124 Bernard E. Saint Jean DriveEast Falmouth, MA 02536-4445T (508) 540–3444 • F (508) 540–8680Online: www.acciusa.comUK Off i ceAssociates of Cape Cod Int’l Inc.Deacon Park, Moorgate RoadKnowsley, Liverpool L33 7RX, United KingdomT (44) 151-547-7444 • F (44) 151-547-7400 Online: www.acciuk.co.ukGerman Off i ceAssociates of Cape Cod PYROQUANT DIAGNOSTIK GmbHOpelstrasse 14, 64546 Mörfelden-Walldorf, GermanyT (49) 61 05-96 10 0 • F (49) 61 05-96 10 15 Online: www.pyroquant.de8PN001443-8 Rev6LAL ReagentsPackagingPyrotell is available in 2 mL or 5 mL MTV and STV sizes. STV’s are sold in packs of ten vials. Certif i cates of Analysis, specif i c to the Pyrotell and CSE lot, can be obtained from ACC or online at www.acciusa.com. It is recommended for use with 0.5 µg/vial Control Standard Endotoxin (E0005).Accessory Products1. LAL Reagent Water, available in multiple package sizes, see page 15 2. Control Standard Endotoxin, E. coli O113:H10, 0.5 µg/vial (E0005)3. Glucashield Buffer (GB051), (1g3)- b - D -Glucan Inhibiting Buffer 4. Pyrosol Buffer with pH indicator (BR051) 5. Pyrosol LAL Reconstitution Buffer (BC051) 6. Pyrotubes ® (TS050), 10 x 75 mm soda lime glass test tubes 7. Pyrotubes (TB240), 12 x 75 mm borosilicate dilution tubes 8. Pyrotubes (TB013), 13 x 100 mm borosilicate dilution tubes 9. 250 µL Pipette tips (PPT25) 10. 1000 µL Pipette tips (PPT10) Multi-Test Vial (MTV), 5 mL/vial (approx. 50 tests/vial)#G5003-5 - - - - - - - - - - - - - - - - - - - - 0.03 EU/mL (5 pack) #G5003-25 - - - - - - - - - - - - - - - - - - 0.03 EU/mL (25 pack) #G5006-5 - - - - - - - - - - - - - - - - - - - - 0.06 EU/mL (5 pack) #G5006-25 - - - - - - - - - - - - - - - - - - 0.06 EU/mL (25 pack) #G5125-5 - - - - - - - - - - - - - - - - - - - 0.125 EU/mL (5 pack) #G5125-25 - - - - - - - - - - - - - - - - - 0.125 EU/mL (25 pack) #G5250-5 - - - - - - - - - - - - - - - - - - - - 0.25 EU/mL (5 pack) #G5250-25 - - - - - - - - - - - - - - - - - - 0.25 EU/mL (25 pack) #GP5003-5 - - - - - - - - - - - - - - - - - - 0.03 EU/mL (5 pack)* #GP5003-25 - - - - - - - - - - - - - - - - 0.03 EU/mL (25 pack)* *must be used with PyrosolMulti-Test Vial (MTV), 2 mL/vial (approx. 20 tests/vial)#G2003-5 - - - - - - - - - - - - - - - - - - - - 0.03 EU/mL (5 pack) #G2003-25 - - - - - - - - - - - - - - - - - - 0.03 EU/mL (25 pack) #G2006-5 - - - - - - - - - - - - - - - - - - - - 0.06 EU/mL (5 pack) #G2006-25 - - - - - - - - - - - - - - - - - - 0.06 EU/mL (25 pack) #G2125-5 - - - - - - - - - - - - - - - - - - - 0.125 EU/mL (5 pack) #G2125-25 - - - - - - - - - - - - - - - - - 0.125 EU/mL (25 pack) #G2250-5 - - - - - - - - - - - - - - - - - - - - 0.25 EU/mL (5 pack) #G2250-25 - - - - - - - - - - - - - - - - - - 0.25 EU/mL (25 pack)Single Test Vial (STV), 0.2 mL/vial,10 vials/pack#GS003 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.03 EU/mL #GS006 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.06 EU/mL #GS125 - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.125 EU/mL #GS250 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.25 EU/mLPyrotell ® Gel-clot Endotoxin TestingGeneral Product DescriptionPyrotell was the fi rst LAL reagent licensed by the US FDA. It is easy to use and is available in both economical Multi-Test Vials (MTVs) and convenient Single Test Vials (STVs). Pyrotell is a robust reagent, producing fi rm, easily read clots and is resiliant to interfering substances. The gel-clot test does not require sophisticated equipment and software and is the simplest LAL test to implement.SensitivityPyrotell is available in a variety of sensitivities: 0.03 EU/mL; 0.06 EU/mL; 0.125 EU/mL; and 0.25 EU/mL.Sample to LAL RatioReconstituted Pyrotell reagent is used at a ratio of 1:1 and a volume of 100 µL/test.Performing the TestFor 2 mL and 5 mL MTV, 100 µL of lysate is mixed with 100 µL of sample in a reaction tube. For STV, 200 µL of sample is added to the vial, which serves as a reaction tube. Test tubes are incubated at 37±1°C for 60 minutes ± 2 minutes. A positive test is indicated if the clot remains solid after the inversion of the test tube. ReconstitutionPyrotell MTV may be reconstituted with LAL Reagent Water (LRW), Pyrosol ® buffer or Glucashield ® buffer, a (1g3)- b - D -Glucan inhibiting buffer, to render the assay endotoxin specif i c. Pyrotell STV is reconstituted by the sample being tested.StabilityOnce reconstituted, Pyrotell MTV is stable for 24 hours, if stored at 2–8°C. Pyrotell MTV may be frozen once and will retain activity for as long as 3 months if stored at or below -20°C. STVs are used immediately upon addition of the sample.For more information please visit our website at www.acciusa.com or contact Customer Service at (508) 540-3444.
