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Corporate HeadquartersAssociates of Cape Cod, Inc.124 Bernard E. Saint Jean DriveEast Falmouth, MA 02536-4445T (508) 540–3444 • F (508) 540–8680Online: www.acciusa.comUK Off i ceAssociates of Cape Cod Int’l Inc.Deacon Park, Moorgate RoadKnowsley, Liverpool L33 7RX, United KingdomT (44) 151-547-7444 • F (44) 151-547-7400 Online: www.acciuk.co.ukGerman Off i ceAssociates of Cape Cod PYROQUANT DIAGNOSTIK GmbHOpelstrasse 14, 64546 Mörfelden-Walldorf, GermanyT (49) 61 05-96 10 0 • F (49) 61 05-96 10 15 Online: www.pyroquant.de6PN001443-6 Rev6LAL ReagentsChromo-LAL Kinetic Chromogenic Endotoxin TestingGeneral Product DescriptionChromo-LAL is lyophilized with substrate reagent and buffers. It is optimized for the kinetic chromogenic LAL test method in microplate readers. Chromo-LAL is a buffered, stable and robust lysate, suitable for quantitative testing of a wide range of samples. The test is read at 405–410 nm.SensitivityThe sensitivity for chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of Chromo-LAL is 0.005 EU/mL.Sample to LAL Ratio Reconstituted Chromo-LAL reagent is used at a ratio of 1:1 and a volume of 100 µL/well (30 tests/vial). Performing the TestThe Chromo-LAL/sample mixture is incubated at 37±1°C and read in a microplate reader at 405–410 nm. Software is used to construct the standard curve and calculate the endotoxin concentrations.ReconstitutionChromo-LAL lysate is reconstituted with LAL Reagent Water (LRW). It can also be reconstituted with Glucashield ® buffer, a (1g3)- b - D -Glucan inhibiting buffer, to render the assay endotoxin specif i c.StabilityOnce reconstituted, Chromo-LAL is stable for 24 hours if stored at 2–8°C. Chromo-LAL may be frozen once and will retain activity for 2 weeks if stored at or below -20°C.PackagingEach vial contains reagent for approximately 30 tests. It is recommended for use with 0.5 µg/vial Control Standard Endotoxin (CSE, E0005). Certif i cates of Analysis, specif i c to the Chromo-LAL and CSE lot, can be obtained from ACC or online at www.acciusa.com.Accessory Products1. LAL Reagent Water, available in multiple package sizes, see page 152. Control Standard Endotoxin, E. coli O113:H10, 0.5 µg/vial (E0005)3. Glucashield Buffer (GB051), (1g3)- b - D -Glucan Inhibiting Buffer 4. Pyrotubes ® (TB013), 13 x 100 mm borosilicate glass dilution tubes5. Pyroplate ® (CA961), 96-well microplate6. 250 µL Pipette tips (PPT25)7. 1000 µL Pipette tips (PPT10)Chromo-LAL 3.2 mL/vial (approx. 30 tests/vial)#C0031-5 - - - - - - - - - - - - - - - - - - - - - - - - - - 5 pack (150 tests) #C0031-25 - - - - - - - - - - - - - - - - - - - - - - - - 25 pack (750 tests)For more information please visit our website at www.acciusa.com or contact Customer Service at (508) 540-3444.