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新医学 2014 年 1月第 45 卷第 1期 49 感染性喉炎和痉挛性喉炎患儿血清中 IL-4、 IFN一 ^ y和 IgE 水平检测的临床意义 薛国昌 任明星周静 月 沈琳娜 宋月娟 夏欢 曹丽 临床研究论著 【 摘要】 目的 探讨感染性喉炎和痉挛性喉炎患儿血清中IL-4、IFN一 和 IgE 水平的变化及临 采用 ELISA 双抗体夹心法检测 26 例感染性喉炎、31例痉挛性喉炎患儿入院时 ( 急性 期) 、出院前 ( 恢复期) 及正常对照组儿童 (25 名) 血清 IL-4、IFN． 水平；采用荧光酶联免疫法检 测 IgE 水平，并进行比较。结果急性期感染性喉炎和痉挛性喉炎患儿血清 IL_4、IgE 水平明显高于 对照组 (P 均 < 0． 05) ；急性期感染性喉炎患儿血清 IgE 水平明显低于痉挛性喉炎患儿 (P < 0． 05) ； 恢复期感染性喉炎和痉挛性喉炎患儿血清 IL-4、leE 水平较急性期明显降低 ( P 均 < 0． 05) ，恢复期 感染性喉炎患儿血清 IgE 水平明显低于痉挛性喉炎患儿 ( P < 0． 05) 。急性期感染性喉炎和痉挛性喉 炎患儿血清IFN一 ． y 水平明显低 于对照组 (P 均 < 0． 05) ；恢复期感染性喉炎和痉挛性喉炎患儿血清 IFN· 水平较急性期明显升高 (P 均 < 0． 05) 。结论乱，IL_4、IFN一 ^ y 和 IgE 在患儿免疫病理机制中起重要作用，检测 IgE 水平有助于两种喉炎的鉴另 q。 【 关键词J 感染性喉炎；痉挛性喉炎；白细胞介素-4；干扰素一 ；免疫球蛋白E 床意义。方法感染性喉炎和痉挛性喉炎患儿存在免疫功能紊 C linical signif i ca nce of IL -4 ，IF N- d I gE in children witlI acute infectious lary ngitis a nd in children witII spasmodic lary ngitis X UE Guo— chang ，R EN 一 xing ，ZHOU J ing-y ue，SE N L in— na ，SONG Yue一 Ⅱ n， XIA Huan， CAO 1 2 De partment o f Pediatrics， the 9th Peo ple's Ho spital o f Wuxi Af f iliated to Suzhou Uni一 ，W uxi 2 1406 2 ，Ch in a 【 Abstract】 IgE level s and thei r cli nic al si gnif i ca n ce in chi ldren with acute infecti ous laryngi ti s and i n chil dren with spas· Object i ve To investigate clinical signif i cance and the change of scram IL- 4 ， IFN一 and modic laryng i tis．M ethods 31 children with spasmodic laryng i tis and 25 hea l thy children．The change of serum IL4 ， IFN一 and IgE levels Serum samples were obtained from 26 children with acute infectious laryng i tis， were o bserved detected by E LISA in Selq lm when hospita l admission ( acute stage) were o bserved． Aand before discharge (conva l escent stage)．E LISA was used to determine the levels o f serum IL 4 ， IFN一 in serum．Serum IgE level was determined wi th enzyme — l inked f l uor o i mmuneassay． R esult s T he l evel s of IL 4 and IgE i n chi l— dren w i th acute infectious laryng i tis and in children w i th spasmodic laryng i tis in acute stage were much higher than that in control group ( P < 0． 05) ．IgE level in children with acute infectious laryng i tis were much lower than that in patients with spasmo d ic laryng i tis ( P < 0． 05 ) ．IL4 and IgE levels in children th acute infec— tious laryng i tis and in children w i th spasmodic laryng i tis in convalescent stage were much lower than these in a— cute stage (P < o． 05)．IgE level in patients with acute infectious laryng i tis in acute stage was lower than that in patients w i th spasmo d ic laryng i tis (P < 0． 05)．T h e levels of IgE in children with acute in f ectious laryng i tis in convalescent stage was lower than that in patients with spasmo d ic laryng i tis ( P < 0． 05 ) ．T h e levels of IF N一 in children with acute infectious laryng i tis and in childr e n w i th spasmo d ic laryng i tis in acute stage were much lo wer than that in control group (P < 0． 05)．IFN一 levels in children with acute in f ectious laryngitis and in children with spasmodic laryngitis in conva l escent stage wer e much higher than these in acute stage ( P< 0． 05) ．Conclusion spasmodic laryngitis．IL4 ，IFN--ra n d IsE might p1．T h ere is immunolo g i c diso rder in infants w i th infectious laryng i tis and in in f ants with dy important role in immunolog i c pathogenesis．Testing the level o f IgE may be valuable in dif f erent diag n osis o f acute in f ectio us laryng i tis and spasmodic laryng i tis． DOI ：10 ． 3969／ g．issn． 0253-9802． 2014 ． 0 1． 013 作者单位： 214062 无锡，苏州大学附属无锡市第九人民医院儿科