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ORIGINAL ARTICLEFirst report of isolation and molecular characterizationof bubaline herpesvirus 1 (BuHV1) from Argentineanwater buffaloesSilvina S. Maidana•Jose´ L. Konrad•Mar? á I. Craig•Osvaldo Zabal•Axel Mauroy•Etienne Thiry•Gustavo Crudeli•Sonia A. RomeraReceived: 16 December 2013/Accepted: 5 June 2014/Published online: 18 June 2014? Springer-Verlag Wien 2014AbstractHerpesviruses have mainly co-evolved withtheir hosts for millions of years. However, bovine herpes-virus 1 (BoHV1) and related ruminant alphaherpesviruseshave been reported to cross the species barrier. Bubalineherpesvirus 1 (BuHV1) is an alphaherpesvirus closelyrelated to BoHV1 and BoHV5. According to the serolog-ical cross-relationships between ruminant alphaherpesvi-ruses, several surveys have studied the occurrence ofBoHV1-related virus infection in wild and domesticruminant species. Recent studies in Argentina showed anincrease in serological prevalence against BoHV1 relatedviruses in water buffaloes (Bubalus bubalis) population.The aim of this study was to investigate the presence ofrelated ruminant alphaherpesvirus in the Argentinean waterbuffalo population. BuHV1 was successfully isolated from5 out of 225 buffaloes analyzed. One isolate was obtainedfrom nasal secretions, and the others were from vaginalswabs. The buffaloes belonged to four different farmslocated in northeastern Argentina. The isolates were char-acterized as alphaherpesvirus by direct immunofluores-cence using FITC-anti-BoHV1 IgG. Restriction analysisperformed with BamHI and BstEII on the complete genomeshowed differences between the isolates and those fromBoHV1 and BoHV5 subtypes. Phylogenetic analysis onboth UL27 and US6 showed similarity in tree topology.While three of the isolates grouped together with sequencesof BoHV5, two other isolates clustered separately. Geneticanalysis of eight concatenated sequences from all isolatesand references strains showed high nucleotide sequenceS. S. Maidana (&) ? M. I. Craig ? O. Zabal ? S. A. RomeraInstituto de Virolog? á, Centro de Investigaciones en Cienciasveterinarias y Agrono´micas (CICVyA), Instituto de tecnolog? áAgropecuaria (INTA), N. Repetto y Los Reseros S/N, CC25,Castelar, B1712WAA Buenos Aires, Argentinae-mail: maidana.silvina@inta.gob.arM. I. Craige-mail: bcraig@cnia.inta.gov.arO. Zabale-mail: ozaval@cnia.inta.gov.arS. A. Romerae-mail: aromera@cnia.inta.gov.arS. S. Maidana ? M. I. Craig ? S. A. RomeraConsejo Nacional de Investigaciones Cient? ´ficas y Tecnolo´gicas(CONICET), Rivadavia 1917,C1033AAJ Ciudad Auto´noma de Buenos Aires, ArgentinaS. S. Maidana ? S. A. RomeraCa´tedra de Inmunogene´tica, Facultad de Ciencias exactas,Qu? ´micas y Naturales, Universidad de Moro´n, Cabildo 134,Moro´n, B1708JPD Buenos Aires, ArgentinaJ. L. Konrad ? G. CrudeliCa´tedra de Teriogenologia, Facultad de Veterinaria, UniversidadNacional del Nordeste, 25 de Mayo 868, 3400 Corrientes,Argentinae-mail: konradjl@hotmail.comG. Crudelie-mail: gacrudelli@hotmail.comA. Mauroy ? E. ThiryVeterinary Virology and Animal Viral Diseases, Fundamentaland Applied Research on Animal Health center and Faculty ofVeterinary Medicine, University of Lie`ge, Boulevard deColonster, 20, B43b, 4000 Lie`ge, Belgiume-mail: amauroy@ulg.ac.beE. Thirye-mail: etienne.thiry@ulg.ac.beS. A. RomeraCa´tedra de Inmunolog? á, Universidad del Salvador, Champagnat1599-Ruta Panamericana-Km 54.5, B1630AHU Pilar,Provincia de Buenos Aires, Argentina123Arch Virol (2014) 159:2917–2923DOI 10.1007/s00705-014-2146-8