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Physicochemical properties of reaginic antibody III. Further studies on the reaginic antibody in rA-globulin preparations Three scra from l,ayu~tcd-scnsiticc patients ICC rc fwkctionatcd by ohvomutogra,phy O,I DEAE cellulose columns and gel filtration. It was corkfifirmed that the waginic a(,- tivity in the chromatographic fractions dicl not pawklZc2 YA-globulin cowxntration. The yA-globulin fractions from the same scra containtd rcaginic a&city. When th yd-globulin prcparrktions toc~‘~~ fwthcr fractionated by chromatography on DEAX ccllulosc columns, the rcaginic: activity UIOS conccntwktctl indo ow of the thyen .sztb- fractions. The pl-ccipitation of yA-globu7in in. the subtraction and the original yd- globulin preparation with rabbit anti-yd-globulin nntibody was not nccompanictl b!y nny detectable dccrcnsc of rw.gi,air acti,rity. The results i?adicatecl that tkc rpaginic activity in the fractiojas ktias laot a.ssociu,td with yA-globulin, but with impztlitics wltich wcrc not dctect?tl by immunodifSzcsio?z methods. ,Thc subfractions of the y-4 qlobuli?a preparntiox from n ragrcccd-sclasitiw patient blocl;ed passive sensitization of normal huma?a &IL zcith rraginic ckntibody of n hrtclologous system. The blocking nctidy of the fractions pa~allclcd the sc~asitizing activity against ragwwd-pollctr extract. It was also foulad that rk ,fraction contccining rcayin but no detectable y,I- ,globztlin, had a dk$Gte blocking nctiaity, 1c11~r~a.s yA-mycloma proteins did not. These findings suggest that both the bloclcin~~ activity mad r(qginic nctirit?y n1.r associa.td with the same prot&, other thfln y~4-~g7ohuli~1. I n previous publications,” ” evidence was presented that rcaginic antibody in two atopic patients’ sera was associated with a unique immunoglobulin other than +-, rA-, y&I-, or yD-globulin. When reagin-containing fractions of the sera were mixed with the antibody specific for yG-, rA-, or yD-globulin to prc- cipitate one of these proteins, essentially all reaginic activity remained in thv supernatant. It was also found that the sedimentation coefficient of t,he reaginic From the Children’s Asthma Research Institute and Hospital, Denver, Colo. Supported by United States Public Health Service Research Grant AT-04985, and in part by contract Nom 3656-12 from the Offices of Naval Rrseawlr. Received for publication Kov. 5, 1965. *Part of the work was done while visiting Associatc~ Professor, I)epartmcnt of Jfrdicinc, University of California, San Franrisro. 108