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405 ANNALES UNIVERSITATIS MARIAE CURIE-SK?ODOWSKA LUBLIN - POLONIA VOL.LX, SUPPL. XVI, 204 SECTIO D 2005 Katedra Nauk Fizjologiczno-Medycznych, Zak?ad Biochemii 1 , Akademia Wychowania Fizycznego Department of Physiological and Medical Sciences, Biochemistry Unit, Academy of Physical Education, Katowice, Szpital Geriatryczny, Katowice 2 Geriatric Hospital, Katowice BARBARA K?APCI?SKA 1 , KATARZYNA KEMPA 1 , JAROS?AW DEREJCZYK 2 , MA?GORZATA MICHALCZYK 1 , Blood antioxidant defense during aging Obrona antyoksydacyjna krwi w procesie starzenia According to the free radical theory of aging (Harman 1991), different types of reactive oxygen species (ROS) generated during normal aerobic metabolism are proposed to be among the most impor-tant contributors to the increased risk of age-related diseases. Blood antioxidant enzymes and vitamins are important components of the complex system of defense against oxidative damage in the organ-ism.This study was aimed at the evaluation of the capacity of the antioxidant defense in centenarians and the elderly subjects. SUBJECTS AND METHODS A total of 165 centenarians (27 male and 138 female) and 82 elderly subjects ( 28 male and 54 fe-male), whose characteristics is presented in Table 1, were enrolled for the study. Venous blood sam-ples were drawn into heparinized test tubes. After their thorough mixing by vortexing the aliquots of 200 µl were transferred into heparinized Eppendorf tubes for subsequent fluorometric assay for Se content according to Danch and Dró?d? (1996). The remaining blood was centrifuged (1000xg, 15 min) to collect plasma and erythrocytes, that were washed three-times with cold (4 o C) saline and kept frozen at -70 o C until being analyzed for activities of antioxidant enzymes, i.e. SOD using a commer-cially available RANSOD kit (Randox Laboratories, UK), CAT by the method of Aebi (1984), GPX with the use of RANSEL (Randox) diagnostic kit and GR according to Glatzle et al. (1970). Plasma samples were assayed for uric acid with the use of a reagent kit (ANALCO), α-tocopherol by using a reverse-phase-HPLC method according to Sobczak et al. (1999) and MDA by the thiobarbituric acid (TBA) test (Buege and Aust 1978) with extraction of the chromogene to n-butanol. Cognitive status of subjects was assessed based on the results of the Mini Mental State Examination (Folstein et al.1975) performed by a geriatrician and a qualified consultant reviewer. The study was performed in compli-ance with the guidelines of the Helsinki Declaration as revised in 1996 regarding the use of human subjects. Statistics Mean and standard deviation (SD) were calculated for all the variables. Between-group differ-ences, with respect to gender and Mini Mental State Examination score as independent variables, were identified with the non-parametric Mann-Whitney U-test. Differences with P values <0.05 were con-sidered significant. In addition, Spearman rank order correlation coefficients were computed to reveal