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www.wjgnet.com wcjd@wjgnet.com 世界华人消化杂志 2010年5月18日; 18(14): 1428-1433ISSN 1009-3079 CN 14-1260/R 基础研究 BASIC RESEARCH内脏高敏感大鼠结肠5-羟色胺转运体蛋白的表达孙程程, 迟 雁, 王化虹, 谢鹏雁孙程程, 迟雁, 王化虹, 谢鹏雁, 北京大学第一医院消化内科 北京市 100034孙程程, 2008年北京大学第一医院消化内科博士, 主要从事消化系统疾病研究.国家自然科学基金资助项目, No. 30600275作者贡献分布: 孙程程、迟雁及王化虹对此文所作贡献均等, 谢鹏雁指导; 此课题由孙程程、迟雁及王化虹设计; 研究过程由孙程程操作完成; 研究所用新试剂及分析工具由迟雁与王化虹提供; 数据分析由孙程程、迟雁及王化虹完成; 本论文写作由孙程程、迟雁及王化虹完成.通讯作者: 王化虹, 教授, 100034, 北京市, 北京大学第一医院消化内科. wanghuahong@medmail.com.cn收稿日期: 2010-03-08 修回日期: 2010-04-21接受日期: 2010-05-10 在线出版日期: 2010-05-18Serotonin transporter expression in rats with chronic visceral hypersensitivity Cheng-Cheng Sun, Yan Chi, Hua-Hong Wang, Peng-Yan Xie Cheng-Cheng Sun, Yan Chi, Hua-Hong Wang, Peng-Yan Xie, Department of Gastroenterology, Peking Univer-sity First Hospital, Beijing 100034, ChinaSupported by: National Natural Science Foundation of China, No. 30600275Correspondence to: Professor Hua-Hong Wang, Depart-ment of Gastroenterology, Peking University First Hospital, Beijing 100034, China. wanghuahong@medmail.com.cnReceived: 2010-03-08 Revised: 2010-04-21 Accepted: 2010-05-10 Published online: 2010-05-18AbstractAIM: To determine whether colonic serotonin transporter (SERT) expression is altered in rats with visceral hypersensitivity and to explore the possible mechanism underlying the pathogenesis of visceral hypersensitivity.METHODS: A rat model of visceral hypersen-sitivity was created by intracolonic injection of acetic acid between postnatal days 8 and 21. The expression of SERT mRNA and protein in the colonic epithelium was detected by RT-PCR and immunohistochemistry, respectively. The levels of serotonin in plasma and colonic tissue were also measured. RESULTS: Colon irritation in neonates re-sulted in chronic visceral hypersensitivity in the absence of identifiable peripheral pathology. Under different capacities the abdominal with-drawal reflex (AWR) score in rats with chronic visceral hypersensitivity was significantly higher than that in controls (P < 0.01). Although plasma serotonin content significantly increased in rats with visceral hypersensitivity compared to controls (95.75 ± 15.99 vs 72.17 ± 8.01, P < 0.01), no significant difference was noted in co-lonic serotonin content between the two groups. SERT immunoreactivity in colonic epithelium was significantly reduced in rats with visceral hypersensitivity (0.187 ± 0.010 vs 0.191 ± 0.011, P < 0.01), whereas a significant increase was noted in SERT mRNA level in the colon of rats with visceral hypersensitivity compared to controls (16.02 ± 3.7 vs 9.47 ± 2.34, P < 0.05).CONCLUSION: In visceral hypersensitivity, the increase in serotonin content is mainly due to decreased degradation of the protein. The expression and function of SERT may be differ-ent in serotonergic neurons compared to colonic epithelial mucosa cells.Key Words: Visceral hypersensitivity; 5-hydrox-ytryptamine; Serotonin transporterSun CC, Chi Y, Wang HH, Xie PY. Serotonin transporter expression in rats with chronic visceral hypersensitivity. Shijie Huaren Xiaohua Zazhi 2010; 18(14): 1428-1433摘要目的: 探讨5-羟色胺转运体蛋白(SERT)在内脏高敏感性中的作用, 为功能性胃肠病的发病机制研究提供理论基础. 方法: 采用乳鼠醋酸灌肠建立大鼠慢性内脏高敏感动物模型, 同时设立对照组. 待乳鼠成年后应用直肠内球囊扩张评估腹壁撤离反射(AWR)的方法, 评估其内脏敏感性; 检测髓过氧化物酶(MPO)评价其肠道黏膜炎症程度; 用RT-PCR方法评价大鼠结肠SERT的mRNA水平, 免疫组织化学方法评价大鼠结肠SERT的表达; 用ELISA方法检测血浆和结肠组织5-HT水平. 结果: 乳鼠醋酸灌肠建立的大鼠慢性内脏高®■背景资料内脏高敏感性被认为是胃肠道功能性疾病最重要的病生理改变之一, 很多研究发现5-羟色胺及其转运体在内脏敏感性升高的病生理机制中发挥重要的作用.■同行评议者黄颖秋, 教授, 本溪钢铁(集团)有限责任公司总医院消化内科