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基于 Hedgehog 通路探究防己诺林碱对三阴性乳腺癌抗肿瘤机制的实验研究张艳辉1 袁王炳高 2 袁邢志博 2 袁王凤梅 2 袁侯红2渊1.青岛大学袁山东 青岛 266042曰2.青岛市中心医院袁山东 青岛 266042冤摘 要院咱目的暂 探究防己诺林碱对三阴性乳腺癌抗肿瘤机制遥 咱方法暂 MTT 法检测防己诺林碱对三阴性乳腺癌细胞的抑制率袁计算 IC 50 曰细胞流式实验观察防己诺林碱对三阴性乳腺癌细胞的凋亡影响曰Western blot 法探究防己诺林碱对 Smo 及 Gli1 蛋白表达的影响遥 咱结果暂防己诺林碱对三阴性乳腺癌细胞 BT549 的活力有影响袁IC 50 值为 8.99滋mol/L遥 随着防己诺林碱浓度升高细胞凋亡率增加袁 三阴性乳腺癌细胞 BT549 通路 Hedgehog 中的 Smo尧Gli1 蛋白表达水平明显降低渊 <0.05冤遥FAN 可以抑制肿瘤的生长遥咱结论暂 防己诺林碱可通过下调细胞凋亡相关信号通路 Hedgehog 来影响三阴性乳腺癌细胞凋亡袁同时影响三阴性乳腺癌肿瘤的生长遥主题词院防己诺林碱曰三阴性乳腺癌曰凋亡曰Hedgehog 信号通路曰肿瘤中图分类号院R737.9 文献标识码院A 文章编号院1671-170X渊2017冤doi院10.11735/j.issn.1671-170X. 2017.Anti鄄tumor Mechanism of Fangchinoline in Triple NegativeBreast Cancer Based on Hedgehog Signaling PathwayZHANG Yan鄄hui 1 袁WANG Bing鄄gao 2 袁XING Zhi鄄bo 2 袁et al.渊1.Qingdao University Medical College袁Qingdao 266042袁China曰2. Qingdao Central Hospital袁Qingdao 266042袁China冤Abstract院咱Objective暂 To explore the anti-tumor mechanism of Fangchinoline in triple negativebreast cancer based on hedgehog signaling pathway. 咱Methods暂 Three negative breast cancer(TNBC) BT549 cells were treated with fangchinoline. The inhibition rate of BT549 cells was de鄄tected by MTT and IC50 was calculated; the cell apoptosis was detected by flow cytometry. Theexpression of Smo and Gli1 protein was detected by Western blot. 咱Results暂 Fangchinoline inhib鄄ited the proliferation of BT549 cells with a IC50 of 8.99滋mol/L. Flow cytometry showed thatFangchinoline induced the apoptosis of BT549 cells in a dose-dependent manner. The expressionof Smo and Gli1 in the BT549 cells was declined with the increasing Fangchinoline concentration.Fangchinoline also inhabited the growth of BT549 breast cancer grafts in tumor-bearing nudemice. 咱Conclusion暂 Fangchinoline inhibits cell growth and enhances cell apoptosis of TNBC cellsin vitro and in vivo袁which may be associated with the regulation by hedgehog signaling pathway.Subject words院Fangchinoline曰triple negative breast cancer曰cell apoptosis曰hedgehog signalingpathways曰tumor通讯作者院王炳高袁副主任医师袁副教授袁硕士研究生导师袁硕士曰青岛市中心医院乳腺诊疗中心袁 青岛市四方区四流南路 127 号渊266042冤曰E鄄mail院wbgqd1965@163.com收稿日期院2016-09-20曰修回日期院2017-01-09三阴性乳腺癌渊 triple negative breast cancer 袁TNBC冤是雌激素受体渊estrogen receptor袁ER冤阴性尧孕激素受体渊progestrone receptor袁PR冤阴性尧人类表皮生长因子受体渊human epidermal growth factor receptor 2袁Her鄄2冤低表达的乳腺癌袁占乳腺癌的 15%~20%袁并且 TNBC 类型发病年龄较其他类型更年轻袁 一般低于 50 岁袁目前治疗手段相对匮乏袁且总生存率较低尧复发率较高 咱1暂 遥 由于 TNBC 细胞具有高增殖特性袁因而抑制增殖信号通路可能会对这类乳腺癌有效遥Hedgehog渊Hh冤信号通路在多种肿瘤组织与细胞系中异常激活袁并与肿瘤的增殖分化尧细胞凋亡尧血管新生尧侵袭转移等密切相关袁阻断肿瘤细胞中 Hh 信号传导通路将为人类肿瘤的治疗提供一个新的有效手段遥 防己诺林碱渊fangchinoline袁FAN冤是从中药防己中提取的一种单体袁 近年来研究显示主要有抗肿瘤尧抗肿瘤细胞多药耐药尧抗病原微生物尧抗神经毒性等作用 咱2暂 袁但其作用机制尚不十分清楚遥 本实验旨1PDF 文件使用 "pdfFactory Pro" 试用版本创建 www.fineprint.cn网络出版时间：2017-05-03 13:17:04网络出版地址：http://kns.cnki.net/kcms/detail/33.1266.R.20170503.1317.006.html