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188宝金博页面版: Regulation of proenkephalin A gene expression in aggregating fetal rat brain cells

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内容提示: Cellular and Molecular Neurobiology, Vol. 11, No. 2, 1991 Regulation of Proenkephalin A Gene Expression in Aggregating Fetal Rat Brain Cells Rabi Simantov 1'3 and Volker H611t 2 Received June 25, 1990; accepted August 31, 1990 KEY WORDS: enkephalin; mRNA; depolarization; gene expression; aggregating cells; neuronal cell culture. SUMMARY 1. Aggregating fetal rat brain cells express a significant amount of pro- enkephalin A (PENK) mRNA, and a selective radioimmunoassay shows that this mRNA is also translated...

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Cellular and Molecular Neurobiology, Vol. 11, No. 2, 1991 Regulation of Proenkephalin A Gene Expression in Aggregating Fetal Rat Brain Cells Rabi Simantov 1'3 and Volker H611t 2 Received June 25, 1990; accepted August 31, 1990 KEY WORDS: enkephalin; mRNA; depolarization; gene expression; aggregating cells; neuronal cell culture. SUMMARY 1. Aggregating fetal rat brain cells express a significant amount of pro- enkephalin A (PENK) mRNA, and a selective radioimmunoassay shows that this mRNA is also translated into enkephalins. 2. Depolarization with potassium chloride (KCI) or veratridine increases the expression of PENK mRNA in a time-dependent fashion, with a maximal increase of sixfold. It is interesting, however, that depolarization of the same cultures with KC1 has no effect on the expression of prodynorphin mRNA. 3. An increase in PENK mRNA levels has been also observed in cultures treated with 8-Br-cAMP, phorbol 12-myristate-13-acetate (TPA), or dexametha- sone. 4. However, incubation of the cultures with the opioid agonist etorphine or the antagonist naltrexone did not alter PENK gene expression, suggesting that there is not feedback control of opioids on PENK biosynthesis in these cells. 5. The increase in PENK mRNA in depolarized and in TPA-, dexamethasone-, or 8-Br-cAMP-treated cultures was not accompanied by a significant increase in the amount of free immunoreactive met-enkephalin. Fetal brain cell cultures are therefore a useful neuronal model system for studying the mechanism that regulated the expression of PENK mRNA. ~Department of Molecular Genetics and Virology, The Weizmann Institute of Science, Rehovot 76100, Israel. 2 Department of Physiology, University of Munich, Pettenkoferstr. 12, D-8000 Mfinchen 2, F.R.G. 3 To whom correspondence should be addressed at Neuroscience Branch, Addiction Research Centre, NIDA, P.O. Box 5180, Baltimore, Maryland 21224. 245 0272-4340/91/0400-0245506.50/0 © 1991 Plenum Publishing Corporation

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