188宝金博页面版

中国环境科学 2020,40(3)：1345~1355 China Environmental Science 左炔诺孕酮对稀有鮈鲫脂质代谢的干扰效应 华江环1* ,史奇朋 2,3 ,郭 威 2,3 ,郭勇勇 2 ,周炳升 2 (1.湖北中医药大学基础医学院,湖北 武汉 430065；2.中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,湖北 武汉 430072；3.中国科学院大学,北京 100049) 摘要：为探究孕激素左炔诺孕酮(LNG)对鱼类脂质代谢的影响,将刚孵化的稀有鮈鲫仔鱼置于 0.8 和 6.5ng/L LNG 暴露 6 个月至性成熟,考察其生长指标、肝脏组织病理学变化、脂质和脂蛋白含量及脂质代谢相关基因的表达情况.结果显示:(1) 0.8,6.5ng/L LNG 暴露显著降低 70dph 稀有鮈鲫的体重和肥满度,且 6.5ng/L LNG 暴露显著降低 180dph 雌、雄鱼的体长或体重;(2) 0.8,6.5ng/L LNG 暴露导致 180dph 成鱼肝脏出现细胞空泡、肝细胞肿胀等损伤;(3) 0.8,6.5ng/L LNG 暴露显著降低 180dph 雌鱼肝脏中甘油三酯、胆固醇的含量,且 6.5ng/L LNG 暴露显著降低雌鱼血浆中甘油三酯的含量,但对雄鱼肝脏及血浆的脂质含量无显著影响;(4) 0.8,6.5ng/L LNG 暴露显著降低 180dph 雌鱼肝脏中脂肪酸合成酶基因(fasn)mRNA 的表达水平,且 6.5ng/L LNG暴露显著降低 180dph 雄鱼肝脏中 fasn 及乙酰辅酶 A 羧化酶基因(acaca) mRNA 的表达水平.由此可见,LNG 长期低剂量暴露可抑制稀有鮈鲫的生长、诱导肝细胞损伤、引起肝脏和血浆脂质含量发生性别差异性变化,并影响肝脏中脂质代谢相关基因的表达,表明 LNG 在低浓度下可干扰稀有鮈鲫的脂质代谢,提示 LNG 对鱼类种群具有较高的潜在生态风险. 关键词：孕激素；左炔诺孕酮；脂质代谢；稀有鮈鲫；长期暴露 中图分类号：X826,X171.5 文献标识码：A 文章编号：1000-6923(2020)03-1345-11 Disrupting effects of levonorgestrel on lipid metabolism in Chinese rare minnow. HUA Jiang-huan 1* , SHI Qi-peng 2,3 , GUO Wei 2,3 , GUO Yong-yong 2 , ZHOU Bing-sheng 2 (1.School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China；2.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China；3.University of Chinese Academy of Sciences, Beijing 100049, China). China Environmental Science, 2020,40(3)：1345~1355 Abstract：In order to explore the effects of progestin levonorgestrel (LNG) on lipid metabolism in fish, newly hatched Chinese rare minnow larvae were exposed to 0.8 and 6.5ng/L LNG for 6months until adults, and the growth parameters, hepatic histopathologic changes, lipids and lipoproteins contents and expression levels of lipid metabolism related genes were investigated. The results showed that the body weights and condition factors of rare minnow at 70dph were significantly decreased by exposure to 0.8 and 6.5ng/L LNG, and the body lengths or body weights were also significantly decreased in females and males at 180dph after exposure to 6.5ng/L LNG. Hepatic damages such as cellular vacuolation and swelling of hepatocytes were induced in adults at 180dph after exposure to 0.8 and 6.5ng/L LNG. The hepatic triglyceride and cholesterol contents in females at 180dph were significantly decreased by exposure to 0.8 and 6.5ng/L LNG, and the serum triglyceride contents were also significantly decreased after exposure to 6.5ng/L LNG, but no effects were observed on male hepatic and serum lipid contents. Besides, the hepatic mRNA expression level of fatty acid synthase gene (fasn) was significantly decreased in females at 180dph by exposure to 0.8 and 6.5ng/L LNG, and the hepatic mRNA expression levels of fasn and acetyl-CoA carboxylase gene (acaca) were also significantly decreased in males at 180dph after exposure to 6.5ng/L LNG. As can be concluded from the results, long-term exposure of rare minnow to low concentrations of LNG inhibited growth, induced hepatic damages, caused sex-specific changes on hepatic and serum lipid contents, and affected mRNA expression of lipid metabolism related genes in the liver. The present study demonstrates that LNG could disrupt lipid metabolism in rare minnow at low concentrations, indicating high potential ecological risks of LNG to fish populations. Key words：progestin；levonorgestrel；lipid metabolism；Chinese rare minnow；long-term exposure 孕激素是广泛应用于人类医药、畜牧养殖业等领域的类固醇类药物,其消费量远高于雌激素或雄激素 [1] .孕激素广泛分布于世界各地各类水环境中,对水生生物的潜在生态环境风险不容忽视 [1- 2] .左炔诺孕酮(LNG)是一种人工合成的孕激素,被检测到存在于全球范围的多种环境介质中 [2,3- 6] ,其中在地表水中的浓度范围一般为 0~38ng/L,最高达 213ng/ L [2,4- 7] . 收稿日期：2019-08-15 基金项目：湖北省自然科学基金资助项目(2018CFB324);湖北省188宝金博页面版厅科学技术研究项目(Q20182010);湖北中医药大学“青苗计划”项目(2017ZZX009) * 责任作者, 讲师, huajianghuan@163.com 。 。. .。 。