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华中农业大学 2008 届硕士学位论文 目 录 目 录...........................................................................................................................0 摘 要...........................................................................................................................1 Abstract..........................................................................................................................3 缩略词（Abbreviation）..............................................................................................5 1 文献综述.................................................................................................................6 1.1 猪水肿病的病原学...........................................................................................6 1.1.1 猪水肿病的发现 ......................................................................................6 1.1.2 产类志贺毒素大肠杆菌的特征 ..............................................................6 1.2 猪水肿病大肠杆菌的毒力因子.......................................................................7 1.2.1 类志贺毒素II型变异体(SLT-IIe).............................................................8 1.2.2 F18ab菌毛 ..............................................................................................11 1.3 猪水肿病流行病学.........................................................................................13 1.4 猪水肿病发病机理及影响因素.....................................................................13 1.4.1 免疫能力 ................................................................................................14 1.4.2 营养水平 ................................................................................................14 1.4.3 遗传抗性 ...............................................................................................15 1.5 猪水肿病的诊断.............................................................................................15 1.5.1 临床症状及剖检病变 ............................................................................15 1.5.2 实验室诊断方法 ....................................................................................16 1.6 猪水肿病的免疫研究....................................................................................17 1.6.1 药物预防 ................................................................................................17 1.6.2 菌体灭活苗 ............................................................................................18 1.6.3 亚单位苗 ................................................................................................18 2 目的和意义...........................................................................................................21 3 材料和方法.............................................................................................................22 3.1 材料.................................................................................................................22 3.1.1 生物材料 ................................................................................................22 3.1.2 血清 ........................................................................................................22 3.1.3 主要试剂 ................................................................................................22 3.1.4 主要试剂配制 .......................................................................................23 3.2 方法.................................................................................................................25 3.2.1 GST-SLT-IIeA蛋白的表达、纯化和鉴定.............................................25 3.2.2 SLT-IIeA间接ELISA检测方法的建立..................................................26 IIIIII