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辽宁大学学 报 自然科学 版 第 30 卷第 1期2003 年 J O UR NA L OF L IA ON IN G UN IV E R S I T Y N at ur al S ciences E d i tion V o1． 3O N o． 1 200 3 S tan da r diz a tion of A d m in istra tion P r oc edur e f or M ice E x posed to C ig ar ette S m ok e and th e C Y P I A 1 G ene E x p ress ion D e tected by an E nz ym e A cti vi ty ZH 0 U R en． qing 。 SHE N Y ing— w az。 ZHOU Y ing— qiao3， B 0 T ao 。 SH A L i 。 fred H A U ( ( 1． T he D epartm ent of L ife S ciences。L iaoning U niversity 。Shenyang 110036。China ； 2 ． Chemical Registration Center of SE PA Beiyuan，Beijing 100012。China； 3 ． T he Ins titute of A pplied E cology 。C h i nese A cademy of S ciences。S henyang 110015，C h i n a ； 4 ． M icrobiology Department。Mich i gan State U n i versity。East L ansing，MI 48824 U ． S ． A ． ) A bstract ： W e set up an ex periment in w hich the conce ntration of cigarette smoke exposed to mice was able to be determine d by detectin g the c o ncentration of solaneso1．T s standardized cigarette-smoke-e x psoin g。 method can ma ke the data fmm dif f erent la boratories be comparable。A t the same time。the pulmonary microsome s of male N M R 1 mic e were used to study the inductiv e e{{eet of tobacco smoke on cytochrome P 450 isoform CY P 1A 1 gene expressio n at the prot d n l ev e1． T he C Y P I A I protein ac t iv i t y Wa s evaluate d by the cataly tic activi ty of 7 - ethoxyresoruf i n O-deethyl a se ( E R0 D ) ． T he re s ults showe d that the pulmonary microsoma l EROD activity Wa s elevate d in the mic e ex posed to cig arette smok e ． K ey words： Sol a nesol；C Y P 1A 1； mouse ； cigarette smok e ； pulmon a ry ． 中囝分类号 ： R378 文献 标识码 ： A 文章编码 ： 1000． 584612003)01． 0001·06 M ice are the most widely used exper i mental mammals in th e study of tobacco smoke tox icology ． H ow ever，no tobac c o smoke c o nc en tratio ns ex posed to mic e have ever been rep o rted in th e related ex． ． periments ．T his situation makes dif ficulties fo r the d ata fro m differen t labo ratories to be com pared with each o ther ．In order to solve this problem ，we set up an expeHmen t in w hich the con cen tration of c igarette smok e ex posed to mice eaR be d etermined by detecting th e conc en tration of s o lan es o 1． C ytoc h rome P 45 0s are micros o mal hemopr o — teins involve d in the biotransfor m ation of exoge- no us preca rc inog en s to a ct iv e c a rcinog e ns[ 1I。A ． mo ng th e c ytoc h r o me P 45 0 s uperfamily， the ex ． pressio n of the pu lmonary C Y P lA l gene in to bac c o smok ers appears to be associated w ith lung can— c e r s1 2 1． T he o ther aim of the pr e sent study is to de— tect the expression of the pulmonary C Y P lA I g ene o f mice exposed t o t o bacc o smok e at the protein level by detection of the ac tiv ity of 7 -ethoxyr e— s o rufin 0 ． deethylase (E R0 D) ．T he results showed that the pulmona r y m icros o ma l E R 0 D activity was increased in the mice ex po sed to cig arette smoke und er the con ditions of o ur lab orato ries ． 1 M A T E R IA L S A N D M E T H O D S 1 ． 1 M aterials an d c hem ic als T he filtere d cigarette ( trade name is S hilin) · 作童堕佥： 周仁清(1 947． )。 男。 大连 人。 教授。 从 事分子生物 学和环境毒理学的研究 收稿日 撕： 2002． 0 8． 30 维普资讯 http://www.cqvip.com