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第 41 卷第 4 期2013 年 7 月DOI:10. 3876/ j. issn. 1000 1980. 2013. 04. 001河 海 大 学 学 报 ( 自 然 科 学 版 )Journal of Hohai University(Natural Sciences)Vol. 41 No. 4Jul. 2013摇 摇 收稿日期: 2012 11 29基金项目: 国家自然科学基金(41001044,41101052);江苏省自然科学基金(BK2011876)作者简介: 赵大勇(1982—)男,河北丰南人,副教授,博士,主要从事湖泊富营养化形成机理及控制技术研究。 E鄄mail: dyzhao@ hhu. edu. cn太湖梅梁湾不同深度沉积物中细菌群落结构组成赵大勇1,孙一萌1,方摇 超1,刘摇 鹏1,曾摇 巾2,王摇 猛1,黄摇 睿1(1. 河海大学水文水资源学院,江苏 南京摇 210098;2. 中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室,江苏 南京摇 210008)摘要: 为研究湖泊沉积物不同深度处的细菌群落结构组成和多样性,选取太湖梅梁湾作为采样点,应用末端限制性片段长度多态性(T鄄RFLP)技术比较湖泊不同深度沉积物样品中细菌群落的结构组成及其多样性。 结果表明:太湖沉积物中细菌具有丰富的多样性,细菌种类和相对丰度与当前已有的可培养细菌研究结果一致。 细菌群落结构随着沉积物深度的增加呈现一定的变化规律,相邻近的沉积物分层中细菌群落结构的相似度较高;随着沉积物深度的增加,沉积物中优势菌属发生了一定的变化;表层沉积物与底层沉积物的细菌群落结构相差较大。 鉴于氧含量随着沉积物深度的增加而逐渐减少,可以推断氧含量的变化是造成湖泊沉积物中细菌群落结构与多样性差异的主要原因。 另外,表层沉积物中的生物扰动也是可能的原因。关键词: 湖泊沉积物;沉积物深度;细菌群落结构;T鄄RFLP 技术;太湖梅梁湾中图分类号:X17;X524摇 摇 摇 文献标志码:A摇 摇 摇 文章编号:1000 1980(2013)04 0283 05Vertical distribution of bacterial community composition insediments of Meiliang Bay in Taihu LakeZHAO Dayong1, SUN Yimeng1, FANG Chao1, LIU Peng1, ZENG Jin2, WANG Meng1, HUANG Rui1(1. College of Hydrology and Water Resources, Hohai University, Nanjing 210098, China;2. State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography andLimnology, Chinese Academy of Sciences, Nanjing 210008, China)Abstract: To investigate the vertical distribution and diversity of bacterial community composition in the sedimentsof Taihu Lake, sediment samples were collected from Meiliang Bay in Taihu Lake. Terminal restriction fragmentlength polymorphism (T鄄RFLP) technology was used to analyze the bacterial community composition and diversityin sediment samples of different depths. High bacterial diversities were observed in the sediments of Taihu Lake,and the bacterial species and relative abundance were found to be consistent with those in previous reports based onthe culture鄄dependent method. Additionally, different T鄄RFLP patterns were observed for samples collected atdifferent depths. Bacterial community compositions derived from those nearby samples showed greater similarities.The dominant bacterial groups varied at different depths. Moreover, the bacterial community compositions of the topand the bottom sediments showed great differences. Dissolved oxygen decreased with the increase of the depth ofsediments, which shows that the variation of dissolved oxygen was the main cause of the differences in bacterialcommunity compositions and diversity. Bioturbation induced by the submerged macrophytes and zoobenthos may beanother cause.Key words: lake sediment; sediment depth; bacterial community structure; T鄄RFLP technology; Meiliang Bay inTaihu Lake浅水湖泊水域较广阔,水深较浅,风浪扰动大,湖泊沉积物与水体物质交换频繁[1]。 以往的研究表明,