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BioMed CentralPage 1 of 9(page number not for citation purposes)BMC NeuroscienceOpen AccessResearch articleApoptosis of supraoptic AVP neurons is involved in the development of central diabetes insipidus after hypophysectomy in ratsYihua Wang†1,2, Cuiping Zhao†3, Zhigang Wang2, Chengwei Wang2, Wenfeng Feng1, Lijin Huang1, Jialin Zhang1 and Songtao Qi*1Address: 1Department of Neurosurgery, Nanfang Hospital of Nanfang Medical University, Guangzhou 510515, Guangdong, PR China, 2Department of Neurosurgery, Second Hospital of Shandong University, Jinan 250033, Shandong, PR China and 3Department of Neurology, Second Hospital of Shandong University, Jinan 250033, Shandong, PR ChinaEmail: Yihua Wang - wangyihua@sdu.edu.cn; Cuiping Zhao - zhaocuipingzsu@126.com; Zhigang Wang - wangzhigangnaf@sdu.edu.cn; Chengwei Wang - doctor_james@126.com; Wenfeng Feng - fengwenfengwei@fimmu.com; Lijin Huang - hhuanglijingfs@fimmu.com; Jialin Zhang - doctor_james@163.com; Songtao Qi* - qisongtao@126.com* Corresponding author †Equal contributorsAbstractBackground: It has been reported that various types of axonal injury of hypothalamo-neurohypophyseal tract can result in degeneration of the magnocellular neurons (MCNs) inhypothalamus and development of central diabetes insipidus (CDI). However, the mechanism ofthe degeneration and death of MCNs after hypophysectomy in vivo is still unclear. This presentstudy was aimed to disclose it and to figure out the dynamic change of central diabetes insipidusafter hypophysectomy.Results: The analysis on the dynamic change of daily water consumption (DWC), daily urinevolume(DUV), specific gravity of urine(USG) and plasma vasopressin concentration showed thatthe change pattern of them was triphasic and neuron counting showed that the degeneration ofvasopressin neurons began at 10 d, aggravated at 20 d and then stabilized at 30 d afterhypophysectomy. There was marked upregulation of cleaved Caspase-3 expression of vasopressinneurons in hypophysectomy rats. A "ladder" pattern of migration of DNA internucleosomalfragments was detected and apoptotic ultrastructure was found in these neurons. There was timecorrelation among the occurrence of diabetes insipidus, the changes of plasma vasopressinconcentration and the degeneration of vasopressin neurons after hypophysectomy.Conclusion: This study firstly demonstrated that apoptosis was involved in degeneration ofsupraoptic vasopressin neurons after hypophysectomy in vivo and development of CDI. Our studyon time course and correlations among water metabolism, degeneration and apoptosis ofvasopressin neurons suggested that there should be an efficient therapeutic window in whichirreversible CDI might be prevented by anti-apoptosis.Published: 25 June 2008BMC Neuroscience 2008, 9:54doi:10.1186/1471-2202-9-54Received: 9 November 2007Accepted: 25 June 2008This article is available from: http://www.biomedcentral.com/1471-2202/9/54© 2008 Wang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.