induced nephropathy (CIN) is one type of AKI and is associated withincreasedmorbidityandmortality.Weanalyzed273patientswitheitherdiabetes or preexisting impaired kidney function getting intra-arterialcontrast media (CM) for coronary angiograms. Blood was taken fordetermination of cystatin C before and 24 h after exposure to intra-arterial CM. Acute kidney injury was defined as the increase of baselinecystatin C of at least 25%. Urinary ET-1 and neutrophil gelatinase-associated lipocalin (N-GAL) concentrations were analyzed in spot urine24 h after CM exposure. 27 patients out of the 273 patients developedCIN. Urinary ET-1 (r = −0.168, p = 0.006) and N-GAL (r = −0.173,p = 0.004) concentrations were inversely correlated with the change ofcystatin C 24 h after CM exposure. Urinary ET-1 concentrations in spoturine of patients without CIN were 0.659 pg/ml and 0.379 pg/ml inpatients with CIN respectively (p = 0.004). Urinary N-GAL concentra-tionsinspoturineofpatientswithoutCINwere19.92 ng/mland8.61 ng/ml in patients with CIN, respectively (p = 0.002). In conclusion, themagnitude of alterations in urinary ET-1 excretion is comparable toalterations of the reference biomarker N-GAL. In contrast to other formsof AKI, urinary concentrations of both biomarkers are reduced indicatingpotentially differences in the underlying pathways leading to kidneydamage after CM exposure as compared to other forms of AKI.doi:10.1016/j.lfs.2014.01.028A predictor to indicate the necessity of measurement ofendothelin and to give the calcium antagonist medicinein outpatientsMinako SekiThe Completion of Doctor Course, International University of Health andWelfare Graduate School, JapanE-mail address: kame-mi@mvd.biglobe.ne.jp (M. Seki)Purpose: This study aimed to examine a predictor to indicate thenecessity of measurement of the endothelin and to give the calciumantagonist medicine of dihydropyridine in outpatients. Method: Theinvestigation was performed on August 25, 2005 to September 19, 2009.The data of patient profile and diagnosis, blood pressure, blood tests, andcurrent medication were collected after obtaining approval of the ethicscommittee for clinical study from 3 hospitals. Result: The effectivenumber of patients for analysis was 376; mean age 59.2 ± 10.4 year.The average clinical values were as follows: weight, 64.4 ± 12.8; BMI,24.8 ± 4.0; SBP, 129.8 ± 17.0mm Hg; DBP,78.3 ± 10.5mm Hg; FPG,119.0 ± 52.9 mg/dl.Finalstepwisemultipleregressionanalysisindicatedpredictor y comprising 4 explained independent variables; age, β.493;weight, β.274; FPG, β.162; SBP, β.158. The Breslow χ2test using theKaplan–Meier command yielded a significant χ210.44 of y both DMandHTinIHD.TheF-testindicatedasignificanceF7.50forywithcalciumantagonist medicine as compared to y without calcium antagonistmedicine (P b 0.01). Conclusion: This study developed the estimator yas a significant predictor to give the calcium antagonist medicine.doi:10.1016/j.lfs.2014.01.029Assessment of circulatory endothelin-1 level among pre- andpost-menopausal rural women in Bangladesh: Result from apopulation-based studyMost. Tanzila Khatuna, Subrina Jesmina,b,c, Arifur Rahmanb,d,AKM Ahsan Habibb,d, Majedul Islama,b, Shamima Aktera,b,c,Nobutake Shimojoa, Sosuke Kimurac, Osamu Okazakic,Masao Moroic, Naoto Yamaguchib, Hidechika Akashic,Farzana Sohaelb,d, Sayeeda Nusrat Sultanab, Sohel Zaedib,c,Saturo Kawanoa, Taro Mizutania, Takashi MiyauchiaaGraduate School of Medicine, Faculty of Medicine, University of Tsukuba,Tsukuba, Ibaraki, JapanbHealth & Disease Research Center for Rural Peoples (HDRCRP),Mohammadpur, Dhaka 1207, BangladeshcNational Center for Global Health and Medicine (NCGM), 1-21-1 Toyama,Shinjuku-ku, Tokyo, JapandShahid Ziaur Rahman Medical College, Bogra, BangladeshE-mail address: jsubrina@gmail.com (A. Rahman)Background: Prevalence of non-communicable diseases is a challeng-ing problem among menopausal women especially in a least developedcountry like Bangladesh, where majority of are women suffering from atleastonechronicdiseaseaftermenopausalage.Themainobjectiveofthisstudy was to determine the circulatory level of endothelin (ET)-1 inBangladeshi pre- and post-menopausal women living in the rural settingand its association with various cardiometabolic risk factors. Methods:This study is based on a community based cross-sectional survey among1802 rural women aged ≥15 years. Plasma level of ET-1 was measuredby ELIZA. Logistic regression was used to estimate the associationbetween circulatory ET-1 level and cardiomatabolic risk factors. Results:ET-1 levels were significantly higher in post-menopausal subjects (post-menopause vs. pre-menopause: 3.92 ± 0.28 vs. 3.28 ±0.14 pg/ml,p = 0.043).Inmultivariableanalyses,wefoundthatET-1had significant positive associations with only plasma cholesterollevel (beta = 0.005, p = 0.012) even after adjusting for age. Metabolicsyndromewaspresentedin25.6%respondentsanditwasmoreprevalentamong post-menopausal (39.3%) as compared to pre-menopausal(16.8%) women. Prevalence of high blood pressure, elevated fastingblood glucose, and high triglyceride were significantly higher in post-menopausal women than in pre-menopausal women (p b 0.05). Meanvalues of systolic blood pressure, plasma levels of triglyceride, HDL,cholesterol and vascular endothelial growth factor were significantlyhigher in post-menopause group compared to pre-menopause group.Conclusions: The higher level of circulatory ET-1 may be associated withhigher prevalence of metabolic syndrome and other cardiometabolic riskfactors among post-menopausal women in many developing countrieslike Bangladesh and proper intervention strategies should be warranted.doi:10.1016/j.lfs.2014.01.030Is excessive blood pressure elevation during resistance exercise arisk factor for arterial stiffening?Takeshi Otsukia, Takahiro KotatobaFaculty of Sport and Health Sciences, Ryutsu Keizai University, Ibaraki,JapanbGraduate School of Sport and Health Sciences, Ryutsu Keizai University,Ibaraki, JapanE-mail address: otsuki-takeshi@rku.ac.jp (T. Otsuki)We previously demonstrated that plasma endothelin-1 (ET-1)concentration is related to resistance exercise-induced arterialstiffening. Additionally, a previous study pointed out that a transientincrease in plasma ET-1 concentration after resistance exercise wascorrelated with blood pressure increase during the exercise. Exaggeratedsystolic blood pressure (SBP) response to exercise is an independentpredictor for future cardiovascular disease. Hence, we hypothesized thatexcessive elevation in SBP during resistance exercise increases arterialstiffness via ET-1 secretion. The aim of this study was to investigate theassociation of arterial stiffness with SBP during resistance exercise. Aftermeasurements of resting hemodynamic parameters, forty-one middle-aged and older subjects performed leg press, a representative resistanceexercise, at 20%, 40% and 60% of their one-repetition maximum. AverageSBP during resistance exercise, age and resting heart rate (HR) wereAbstractse68
