188宝金博页面版

中国农业科学 2011,44(3):447-455 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.03.002 收稿日期： 2010-06-29； 接受日期： 2010-10-26 基金项目： 转基因生物新品种培育科技重大专项（2008ZX08003-002）、 河北省“十一五” 科技支撑计划（06220108D-2） 联系方式： 马 磊， E-mail： malei181@yahoo.com.cn。 李 盼， E-mail： lipan0205@163.com。 马磊与李盼为同等贡献作者。 通信作者陈景堂， Tel：0312-7528108； E-mail： chenjingtang@126.com 低植酸玉米自交系的筛选及其遗传分析 马 磊, 李 盼, 陈 哲, 赵永锋, 祝丽英, 黄亚群, 陈景堂 （河北农业大学农学院/国家玉米改良中心河北分中心/“华北作物种质资源研究与利用” 省部共建188宝金博页面版部重点实验室， 河北保定 071001） 摘要： 【目 的】 筛选获得低植酸玉米自 交系， 研究低植酸性状的遗传规律， 为低植酸玉米杂交种的选育提供依据。 【方法】 通过钼酸铵显色法对 100 份玉米自 交系进行测定， 将筛选得到的低植酸自 交系鲁原 92 与已知低植酸突变体 Lpa/lpa241、 Lpa/Lpa241 和齐 319 进行杂交， 分析低植酸性状的遗传； 并用钼酸铵显色法和植酸含量国标测定法对 6 个不同来源的齐 319、 鲁原 92 及其杂交种进行植酸含量比较分析， 同时通过发芽试验对齐 319、 齐319× Lpa241/lpa241、 鲁原 92× Lpa241/lpa241、 鲁原 92× 齐 319 苗期表现进行鉴定分析。【结果】 钼酸铵显色法显示， 鲁原 92 的无机磷含量达到 0. 93 µg·mg-1水平， 初步确定属于低植酸自 交系； 其低植酸性状受隐性单基因控制， 可能与 lpa241 等位； 齐 319 在植酸性状上表现杂合性， 存在苗期纯合致死现象； 齐 319× Lpa241/lpa241、鲁原 92× Lpa241/lpa241、 鲁原 92× 齐 319 F2苗期纯合致死比例相近； 不同来源的同一自 交系间植酸含量存在显著差异。 【结论】 筛选得到低植酸自 交系 1 个， 分析其低植酸性状为隐性单基因控制， 且与 lpa241 等位。 关键词： 玉米； 自 交系； 低植酸； 遗传 Genetic Analysis and Identification of Maize (Zea mays L.) Low Phytic Acid Inbred Lines MA Lei, LI Pan, CHEN Zhe, ZHAO Yong-feng, ZHU Li-ying, HUANG Ya-qun, CHEN Jing-tang (College of Agronomy, Agricultural University of Hebei/Hebei Sub-center of Chinese National Maize Improvement Center/Northern China Key Laboratory for Crop Germplasm Resources of Ministry of Education, Baoding 071001, Hebei) Abstract: 【Objective】 The purpose of this experiment was to provide a theoretical basis for selective breeding of the low phytic acid maize hybrid through screening low phytic acid materials from general maize inbred lines and study the genetic characteristics of low phytic acid trait. 【Method】 In this study, through using the colorinmetric assay for high inorganic phosphorus (HIP), kernels from 100 maize inbred lines were indirectly screened respectively. To analyze the genetic characteristics of low-phytic acid trait, the screened Luyuan 92 was hybridized with known low phytic acid mutants Lpa/lpa241, Lpa/Lpa241 and low-phytic acid inbred line Qi 319. Also the phytic acid content of two inbred lines Qi 319, Luyuan 92 and their reciprocal hybrids from six different origins were analysed by modified colorimetric assay and GB technique. The phenotypes of Qi 319, Luyuan 92×Qi 319, Qi 319×Lpa241/lpa241, Luyuan 92×Lpa241/lpa241 seedlings were investigated through germination test.【Result】 The inorganic phosphorus content of Luyuan 92 was about 0.93 µg·mg-1, which was higher than that of other materials. According to the screening results of F1 seeds between mutants (L/lpa241) and Qi 319, Luyuan 92, the segregation ratio of HIP type and wild type fit into 1﹕ 1 respectively, which indicated that its low phytic acid character was controlled by single recessive gene, and was possibly allelic with lpa241. The phytic acid content of the same inbred line from six different origins displayed a significant difference. Qi 319 represented heterozygosity in low phytic acid character and lethal recessive homozygous genotype at seedling stage. 【Conclusion】In this study, one maize inbred line with low phytic acid content was obtained. Its low phytic acid trait was predicted preliminarily to be controlled by single recessive gene and was possibly allelic with lpa241.