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生 态 学 报 2011， 31( 3) :0793—0800Acta Ecologica Sinicahttp: / /www． ecologica． cn基金项目: 国家 “十一五” 科技攻关项目子课题资助项目( 2006BAC01A15) ; 中国科学院知识创新工程重大项目( KZCX2- XB2-02-01-03)收稿日期:2009-12-20;修订日期:2010-06-13* 通讯作者 Corresponding author． E- mail: pankw@ cib． ac． cn．阿魏酸、 对羟基苯甲酸及其混合液对土壤氮及相关微生物的影响母容1，潘开文1， *，王进闯1，陈其兵610041; 2． 四川农业大学风景园林学院，成都2( 1． 中国科学院成都生物研究所 生态恢复重点实验室，成都611100)摘要: 通过室内培养法， 研究了不同浓度的阿魏酸、 对羟基苯甲酸及其混合液对土壤氮素、 与氮素转化相关的微生物和酶的影响。结果表明， 10－4mol/L 阿魏酸和对羟基苯甲酸使土壤铵态氮降低了 11． 18% 和 10． 87%， 硝态氮降低了 6． 33% 和 3． 95%;10－3mol/L 阿魏酸、 对羟基苯甲酸及其混合液分别使可溶性有机氮降低了 6． 59%、 10． 16% 和 10． 39%。阿魏酸、 对羟基苯甲酸及其混合液抑制了氨化细菌、 硝化细菌和反硝化细菌的生长， 削弱了土壤脲酶与蛋白酶的活性。与对照相比， 10－4mol/L 混合液降低了 26． 04%的氨化细菌、 30． 79%的硝化细菌和 16． 74% 的反硝化细菌。10－3mol/L 阿魏酸减少了 3． 33% 的土壤脲酶和20． 87%的蛋白酶活性; 10－3mol/L 对羟基苯甲酸降低了土壤脲酶 6． 63%， 蛋白酶 22． 94%; 10－3mol/L 混合液减少了土壤脲酶7． 47%和蛋白酶 23． 79%。混合液对土壤氮素转化的抑制作用最强， 表明阿魏酸和对羟基苯甲酸存在协同作用。阿魏酸和对羟基苯甲酸等酚酸类化合物通过抑制土壤氮素转化微生物及其酶活性， 从而影响土壤氮素转化。关键词: 阿魏酸; 对羟基苯甲酸; 土壤 N 素; 抑制作用; 协同作用Effects of ferulic acid，p- hydroxybenzoic acid and their mixture on mineralnitrogen and relativemicrobial function groups in forest soilsMU Rong1，PAN Kaiwen1， *，WANG Jinchuang1，CHEN Qibing21 ECORES Laboratory，Chengdu Institute of Biology，Chinese Academy of Sciences，Chengdu 610041，China2 College of Landscape Architecture，Sichuan Agricultural University，Chengdu 611100，ChinaAbstract: Phenolic compounds is an important class of plant secondary metabolites，whose molecular structure contains atleast one hydroxyl connected directly to the benzene ring on the aromatic compounds． Phenolic compounds in the soils fromthe decomposition of plant parts and their residues play an important role in soil nitrogen ( N) transformations by alteringsoil microbial activity． Previous studies of effects of phenolic compounds on soil N transformations and relative microbialactivities have focused on macromolecular phenolic compounds such as tannic acids． However，the effects of lowermolecular weight phenolic compounds such as ferulic acid and p- hydroxybenzoic acid on soil N transformations are not wellunderstood． In this paper，concentrations of 10－4mol/L and 10－3mol/L of ferulic acid，p- hydroxybenzoic acid and theirmixture were respectively added to forest soils for investigating the effects of phenolic compounds on soil mineral N，microbes and enzyme activity within one month incubation under room temperature．The results showed that concentrations of NH+4- N，NO－3- N and dissolved organic N ( DON) were lower in the soilsamended with different phenolic acids than those without any such amendment． NH+4- N was respectively reduced by 11. 2%and 10． 9% in the soils with the addition of 10－4mol/L ferulic acid and p- hydroxybenzoic acid． The addition of these ferulicacid and p- hydroxybenzoic acid lowered the soil NO－3- N by 6． 3% and 4． 0%． The addition of 10－3mol/L ferulic acid，p-hydroxybenzoic and the mixture decreased DON by 6． 6%，10． 2% and 10． 4%，respectively． Ammonifiers，nitrifiers，